Coding
Part:BBa_K1414002
Designed by: keisuke sugahara Group: iGEM14_KAIT_JAPAN (2014-10-07)
IL5Ra+HRV Cleavage Sequence+AraC
This part is Interleukin 5 receptor alpha subunit (IL5Ra) fused to AraC protein.IL5Ra and AraC are connected by HRV cleavage sequence. IL5Ra is membrane protein.This protein specifically bind to Interleukin 5 and form hetero dimer with IL5 beta subunit. HRV cleavage sequence is Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro peptid sequence. HRV protease is highly specific for this peptid sequence and cleave after the glutamine residue.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 186
Illegal PstI site found at 304 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 304
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1149
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 186
Illegal PstI site found at 304 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 186
Illegal PstI site found at 304 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 368
Illegal BsaI.rc site found at 1237
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Categories
Parameters
None |